Figure 4

Reduced social interaction of Casp3^−/− male mice in freely moving reciprocal social interaction assays

(A) Latency to make the first contact with the other mouse. (B) Time spent engaged in male-female social contacts during 10 min (p = 0.0001 for genotype, p = 0.0112 for session, p = 0.1111 for genotype × session, by repeated measures two-way ANOVA). (C) Number of male to female mountings during 10 min (p = 0.0008 for genotype, p < 0.0001 for session, p = 0.0002 for genotype × session, by repeated measures two-way ANOVA). (D) Time spent engaged in social contacts between a pair of adult male and juvenile C57BL/6 male mice during 10 min. (E) Time spent engaged in female-female social contacts during 10 min. (F) Relationship between time spent engaged in male-female social contacts and total distance traveled by both mice during 10 min in session 1. (G) Relationship between time spent engaged in male-female social contacts and number of male to female mountings during 10 min in session 1. (H) Correlation between total distance traveled by both mice in session 1 and session 2. (I) Correlation between time spent engaged in male-female social contacts in session 1 and session 2. Graphs show mean ± SEM in (A)-(E), for 20 WT males, 20 Casp3^−/− males, 20 WT females and 19 Casp3^−/− females. *p < 0.05, **p < 0.01, ***p < 0.001, for genotype effect by ANOVA in (A), (D) and (E), or by Bonferroni post tests following two-way ANOVA with the factors of genotype and session in (B) and (C).