Figure 7

(a) Schematic representation of the cell culture models investigated in this study (This figure was drawn by Tzu-Keng Chiu). (b) Fluorescent microscopic photographs of MSCs cultured in the 4 culture models studied [(I) Static 2-D cell culture; (II) Perfusion 2-D cell culture; (III) Static 3-D cell culture; and (IV) Perfusion 3-D cell culture] on days 8 (upper row) and 16 (lower row); the green and red images represent live and dead cells, respectively. (c) Proliferation curves of MSCs cultured in the cell culture models tested; significant differences are indicated by stars (^**p<0.01, ^*p<0.05, ANOVA). Inset: Regression lines of the proliferation curves; the slope of each regression lines represents the cellular proliferation rate (% day⁻¹).