Figure 3 | Scientific Reports

Figure 3

From: Subsite-specific contributions of different aromatic residues in the active site architecture of glycoside hydrolase family 12

Figure 3

Isothermal titration calorimetry and thermodynamic parameters determined.

The raw binding heat levels of (a) TrCel12A-E200Q with the concentration of protein 10.6 μM and that of ligand 22.0 μM, (b) TrCel12A-W7Y:E200Q with [protein] = 6.40 μM, [ligand] = 22.0 μM and (c) TrCel12A-W22Y:E200Q with [protein] = 11.5 μM, [ligand] = 27.5 μM.

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