Table 1 Kinetic parameters of TrCel12A and the tyrosine mutations to CMC.
Enzyme | Km(g/L) | kcat (1/s) | kcat/Km (L·g−1·s−1) |
|---|---|---|---|
TrCel12A | 22.74 | 277.8 | 12.21 |
TrCel12A-W7Y | 12.05 | 168.3 | 13.96 |
TrCel12A-W22Y | 13.16 | 72.06 | 5.48 |
Enzyme | Km(g/L) | kcat (1/s) | kcat/Km (L·g−1·s−1) |
|---|---|---|---|
TrCel12A | 22.74 | 277.8 | 12.21 |
TrCel12A-W7Y | 12.05 | 168.3 | 13.96 |
TrCel12A-W22Y | 13.16 | 72.06 | 5.48 |