Figure 1 | Scientific Reports

Figure 1

From: Chronic Metabolic Acidosis Activates Renal Tubular Sodium Chloride Cotransporter through Angiotension II-dependent WNK4-SPAK Phosphorylation Pathway

Figure 1

Expression of NCC and effect of hydrochlorothiazide (HCTZ) in WT and WTA mice.

(A) Semiquantitative immunoblotting of total kidney extracts indicate that total and p-NCC (T58 and S71) were significantly enhanced in WTA mice (6 mice/group, 3 separate gels). The blots and gels were cropped and the full-length blots/gels are presented in Supplementary Fig. S1. (B) Reverse transcriptase PCR and real-time PCR indicated similar levels of NCC transcripts in WTA and WT mice (4 mice per group). The blots and gels were cropped and the full-length blots/gels are presented in Supplementary Fig. S2. (C) Following a single dose of HCTZ, WTA mice produced more urine and had higher FeNa (8 mice per group). *p < 0.05 vs. WT mice.

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