Figure 2 | Scientific Reports

Figure 2

From: Chronic Metabolic Acidosis Activates Renal Tubular Sodium Chloride Cotransporter through Angiotension II-dependent WNK4-SPAK Phosphorylation Pathway

Figure 2

Expression of SPAK and WNK4 in WT and WTA mice.

(A) Semiquantitative immunoblotting of total kidney extracts indicated increased expression of total SPAK, p-SPAK and WNK4 in WTA mice (6 mice/group, 3 separate gels). The blots and gels were cropped and the full-length blots/gels are presented in Supplementary Fig. S3. (B) Immunofluorescence staining of NCC and p-NCC in the DCTs indicated total and p-NCC were more luminally condensed with greater expression of SPAK and WNK4 in WTA mice. *p < 0.05 vs. WT mice.

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