Figure 5

Effect of acidosis on the Na⁺ balance and expression of NCC.

(A) WTA mice experienced early natriuresis, but this abated after day 5 (upper panel), leading to a negative Na⁺ balance before day 3 and a positive balance thereafter (lower panel). WTA mice given losartan (WTA-L mice) had high urinary Na⁺ and a negative Na⁺ balance on day 5 and this differed significantly from WTA mice given spironolactone (WTA-S mice) (6 mice/group). (B) At day 3, the levels of total and p-NCC were similar between WT and WTA mice, but WTA mice had significantly higher levels of NCC expression on day 5 (6 mice/group, 2 separate gels). *p < 0.05 vs. WT mice; ^#p < 0.05 vs. WTA-S mice. The blots and gels were cropped and the full-length blots/gels are presented in Supplementary Fig. S6.