Figure 1
From: A white spot syndrome virus microRNA promotes the virus infection by targeting the host STAT
Roles of viral miRNA in virus infection in shrimp in vivo.
(A) The expression profile of WSSV-miR-22 in WSSV-infected shrimp. Northern blotting was conducted using gills of WSSV-infected shrimp at different time points. U6 was used as a control. The numbers indicated the time points post-infection. (B) The effect of WSSV-miR-22 overexpression on the virus infection in shrimp. To overexpress the viral miRNA, WSSV-miR-22 and WSSV were co-injected into shrimp. At different time post-infection, the WSSV copies in shrimp were examined. U6 was used as a control. (C) The influence of WSSV-miR-22 silencing on the virus infection in vivo. AMO-WSSV-miR-22 and WSSV were co-injected into shrimp. At various time post-infection, the WSSV copies in shrimp were evaluated. The numbers showed the time points after WSSV infection in shrimp. (D) The affect of WSSV-miR-22 overexpression on the WSSV ie1 gene expression. WSSV and WSSV-miR-22-mimic or control-miRNA were co-injected into shrimp. At different time points post-infection, the expression of ie1 was examined with quantitative real-time PCR. (E) The impact of WSSV-miR-22 silencing on the ie1 expression in vivo. The ie1 mRNA level in WSSV-miR-22-silenced shrimp was evaluated with quantitative real-time PCR. In all panels, asterisks indicated significant differences (*p < 0.05; **p < 0.01) between treatments.
