Figure 3
From: A white spot syndrome virus microRNA promotes the virus infection by targeting the host STAT
The role of host STAT in virus infection.
(A) The distribution of STAT in various tissues of shrimp. The shrimp STAT mRNA levels of hemocyte, heart, hepatopancreas, gill, intestine and stomach were examined using quantitative real-time PCR. Shrimp actin was used as a control. (B) The expression profile of STAT in shrimp in response to virus infection. Shrimp were infected with WSSV. At different time post-infection, the STAT expression in gills was detected with quantitative real-time PCR. (C) The silencing of the STAT expression in shrimp. The sequence-specific STAT-siRNA was injected into shrimp to knock down the expression of STAT. As a control, STAT-siRNA-scrambled was included in the injections. WSSV alone was used as a positive control. (D) The influence of STAT silencing on virus infection. The WSSV copies in gills of STAT-siRNA-treated shrimp were quantified using quantitative real-time PCR. In all panels, statistically significant differences between treatments were indicated by asterisks (**p < 0.01).
