Figure 4
From: A white spot syndrome virus microRNA promotes the virus infection by targeting the host STAT
The influence of host JAK gene on virus infection.
(A) The expression profile of JAK in shrimp tissues. The mRNA level of JAK was determined using quantitative real-time PCR. The shrimp action was used as the control. (B) The detection of JAK expression in gills of WSSV-challenged shrimp. PBS was used as a control. (C) The silencing of JAK expression in shrimp. The sequence-specific siRNA (JAK-siRNA) was injected into WSSV-infected shrimp to knock down the expression of JAK. At different time post-infection, the JAK expression was examined with quantitative real-time PCR. As a negative control, JAK-siRNA-scrambled was included in the injections. WSSV alone was used as a positive control. (D) The effect of JAK silencing on virus infection. The WSSV copies in gills of siRNA-treated shrimp were evaluated using quantitative real-time PCR. In all panels, statistically significant differences between treatments were indicated by asterisks (**p < 0.01).
