Figure 5
From: A white spot syndrome virus microRNA promotes the virus infection by targeting the host STAT
The effects of host TEP1 and TEP2 on virus infection.
(A) Tissue distributions of TEP1 and TEP2 in shrimp. The mRNA levels were determined using quantitative real-time PCR. The shrimp action was used as a control. (B) The expression analysis of TEP1 and TEP2 in shrimp in response to the virus challenge. Shrimp were infected with WSSV. At different time post-infection, the mRNA levels of TEP1 and TEP2 in shrimp were evaluated with quantitative real-time PCR. (C) The gene expression silencing of TEP1 and TEP2 in shrimp. The sequence-specific TEP1-siRNA or TEP2-siRNA was injected into shrimp. Then the gene expression was examined. As a negative control, siRNA-scrambled was included in the injections. WSSV alone was used as a positive control. (D) The detection of WSSV copies in shrimp. The gills of siRNA-treated shrimp were subjected to the quantitative real-time PCR analysis to examine the WSSV copies. WSSV alone was used as a positive control. In all panels, asterisks indicated significant differences between treatments (**p < 0.01).
