Figure 5

(A) CVs of (a) bare electrode and (b) Mb/Au-PTy-f-MWCNT/GCE, with added different concentrations of NaNO2 (b–o) 0.4–10 mM in pH 7.0 buffer solution (from inner to outer); scan rate: 50 mV/s. (B) Amperometric response curves of Mb/Au-PTy-f-MWCNT-modified rotating electrode upon successive addition of NaNO2 into pH 7.0 PBS solution. Applied potential: +0.74 V. Inset: calibration curve of steady-state currents vs. NaNO2 concentration of Mb/Au-PTy-f-MWCNT biocomposite. (C) CVs of (a) bare electrode and (b–e) Mb/Au-PTy-f-MWCNT/GCE in PBS free of H2O2 with various concentrations from 0 to 4 mM in N2-saturated PBS (0.05 M, pH 7). (D) The amperometric response of the Mb/Au-PTy-f-MWCNT-modified rotating electrode for the successive additions of H2O2 (conditions: −0.3V constant potential, pH 7.0, rotation speed 1200 rpm).