Figure 5
From: Identification of a highly efficient stationary phase promoter in Bacillus subtilis
Site-directed mutagenesis from bp −48 to −1 of the Pylb promoter.
The DNA sequence of wild-type Pylb is shown at the top (WT). The transcription start site (TSS) of Pylb was identified by CR-RT-PCR. The mutated nucleotides of the M4843 to M0301 mutants are shown below the WT promoter sequence. Green fluorescence intensity was determined using a microplate reader. The fold-changes in Flu/OD600 (fluorescence units per OD600) relative to the WT (set to 100%) indicate the transcriptional activities of the mutants. The significant fold-changes in strains M3833, M3328, M1308 and M0803 were due to large reductions in fluorescence intensity. Data are averages of three independent experiments, each of which comprised four replicates.
