Figure 6
From: Identification of a highly efficient stationary phase promoter in Bacillus subtilis
Pullulanse and OPHC2 activities of B. subtilis strains harboring various promoters after 18 h of culture.
(A) Production of pullulanse by B. subtilis WB600 harboring the recombinant plasmid Pylb-ZDs-Pul-pUBC19 (indicated by Pylb-Pul), P43-ZDs-Pul-pUBC19 (P43-Pul), or no recombinant plasmid (WB600) after 18 h of culture. (B) SDS-PAGE analysis of pullulanse activity in the culture supernatant that was concentrated about 15 folds after 18 h of culture. M: protein molecular mass marker. 1: B. subtilis WB600 as a negative control. 2 and 3: Pylb-Pul-1, Pylb-Pul-2. 4 and 5: P43-Pul-1 and P43-Pul-2 and the arrows indicate target protein pullulanse. (C) Production of OPHC2 by B. subtilis WB600 harboring the recombinant plasmid Pylb-ZDs-OPHC2-pUBC19 (indicated by Pylb-OPHC2), P43-ZDs-OPHC2-pUBC19 (P43- OPHC2) and no recombinant plasmid (WB600) after 18 h of culture. (D) SDS-PAGE analysis of OPHC2 in the culture supernatant that was concentrated about 15 folds after 18 h of culture. M: protein molecular mass marker. 1: B. subtilis WB600 as a negative control. 2 and 3: Pylb-OPHC2-1, Pylb-OPHC2-2. 4 and 5: P43-OPHC2-1 and P43-OPHC2-2 and the arrows indicate the target protein OPHC2. Data are averages of three independent experiments.
