Figure 2
From: Evolution of cellular morpho-phenotypes in cancer metastasis
Visually-aided morpho-phenotyping recognition (VAMPIRE) analysis.
(A) Demonstration of VAMPIRE analytical processes. (B–D) Heat maps show the probability of cells in each nucleus and cell shape modes (P(NSk) or P(CSk)) for each different sample (B,C). Heat maps show the population distributions of different paired nuclear and cellular shapes modes (P(NSk&CSj)) for different samples (D). Color coding (blue to red) corresponds to low and high occurrence. (E) A panel of five heterogeneity properties, including CV of nucleus (CV(RN)) and cell size (CV(RC)), entropy of nucleus (S(NS)) and cell shape (S(CS)) and entropy of paired nucleus-cell shape (S(NS&CS)) was used to represent overall heterogeneity profiles of cell morphology of 9 PT and LM samples. The magnitude of heterogeneity of these cell populations is shown in a heat map where color from red to green indicates increasing degree of heterogeneity. (F) Images of PAC01 cells and PAC09 cells. Nuclei and F-actin are labeled in blue and green.
