Figure 5 | Scientific Reports

Figure 5

From: Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement

Figure 5

Fed-batch HCDF characterization of 1,000-L scale.

(a) Profiles of glycerol feeding rate, methanol feeding rate, DO level, temperature and pH during the whole process. (b) Air pressure, agitation and aeration profiles. (c) Cell density (DCW) profile. (d) Specific growth rate (μ, h−1) profile. (e) Volumetric enzyme activity (U/L) and biomass enzyme activity (U/g) profiles. (f) Volumetric enzyme production (g/L) and biomass enzyme production (mg/g) profiles. (g) Specific production rate (qP) profile. Data are means of three parallel measurements.

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