Figure 2 | Scientific Reports

Figure 2

From: An ancestral host defence peptide within human β-defensin 3 recapitulates the antibacterial and antiviral activity of the full-length molecule

Figure 2

The γ-core peptides have potent antimicrobial activity without affecting cell viability.

Antibacterial activity of peptides γ,δ,ε in comparison with full-length HBD3 on (A) E. coli. and (B) P. aeruginosa and (C) S. aureus at the concentration of 2.5 μM (blue bars) and 12.5 μM (red bars), in the presence of the indicated concentrations of NaCl. Error bars show the standard deviations (SDs) from three independent experiments. Based on the absence of activity on E. coli, peptide δ was not tested on the other species. (D) Effects of the indicated peptides tested at the indicated concentration on A549 cells viability by MTT test. The data are expressed as the means ± standard error of three independent experiments. (E) Effects of the γ-core peptides on HIV infectivity. The γ-core peptides γ and ε were tested in oxidized and reduced form on the indicated HIV-1 viral strains at the concentration of 1, 5, 10, 20, 50, 100 and 150 μM, in comparison with reduced HBD3. Data expressed as % neutralization are from triplicates, with indicated SD. The same data in Table format are in the S.I. See also Tables S6, S7.

Back to article page