Table 1 Enhancement of Lactoferrin uptake in cells by GAPDH.

From: Secreted multifunctional Glyceraldehyde-3-phosphate dehydrogenase sequesters lactoferrin and iron into cells via a non-canonical pathway

Cell type

Fold change in Lactoferrin MFI of cells internalizing both GAPDH and Lf vs. only lactoferrin +ve cells

Mouse Enterocytes

5.52*

Mouse Hepatocytes

4.28*

Mouse Bone Marrow cells

3.7*

Mouse Spleen Macrophages

2.1*

Mouse Peritoneal Macrophages (Ex vivo)

1.17*

Mouse peritoneum cells (In-Vivo)

6.1*

Human Lymphocytes

1.27*

J774

2.62*

PMA activated THP1

2.07*

Non activated THP1

No significant uptake of Lf

CHO

4.95*

CHO-TRVb

2.83*

K562

1.6*

Neuro-2A (N2A)

4.02*

  1. Relative trafficking of Lf by sGAPDH as compared to surface receptors. 5 × 105 cells were incubated at 37 °C for 30 minutes with either; (i.) only lactoferrin Alexa-633 (5 μg), (ii.) only GAPDH-FITC (10 μg) or (iii.) both (i.) & (ii.). After internalization cells were washed, pronase treated and analyzed by FACS. A two-color quadrant analysis was done to see relative trafficking as described in methods, *p < 0.001 compared to Lf internalized via surface receptors only. n = 104 cells in each case.
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