Figure 1
From: SMC1B is present in mammalian somatic cells and interacts with mitotic cohesin proteins
Smc1b is expressed in both germ and somatic mouse cells.
(A) In addition to testes, Smc1b is also transcribed in brain, spleen and heart mouse tissues when analyzed by RT-qPCR. No expression was detected in kidney, liver and Smc1b−/− testis (as negative control). (B) The specificity of 308–432 polyclonal antibody was assayed using protein extracted from different Smc1b knock-out mouse tissues. No signal was detected in testis, heart, kidney, spleen or liver. A clear signal was visualized in wild-type mouse tissue. (C) Smc1b RNA is also translated and bands are visualized in brain, heart and spleen, in addition to reproductive tissues (testis and ovary). No band observed in kidney, liver or Smc1b−/− testis. An antibody against tubulin was used as loading control. (D) SMC1B co-IPed with SMC3 and RAD21 in testis, brain, heart and spleen whereas it did not co-IP in Smc1b−/− testis or without no antibody (no Ab). Total protein extract from wild-type testis tissue was used as positive control. SMC1A does not co-IP with SMC1B. (E) SMC3 was found to be co-precipitated with both SMC1A and SMC1B. Western blot images regarding flow-through are in Supplementary Fig. S3.
