Figure 2
From: SMC1B is present in mammalian somatic cells and interacts with mitotic cohesin proteins
SMC1B is expressed in human fibroblasts.
(A) Total protein extracts from wild-type testis tissue and Smc1b knockout testes were respectively used as positive and negative controls. (B) SMC1B was found to interact physically with SMC3 cohesin factor by IP also in human fibroblast whereas no signal was detected for SMC1A. Total testis and fibroblast extracts were used as control. The rabbit polyclonal antibody against the fragment 308–432 was used. (C) Western blotting showing the downregulation of SMC1B in human fibroblasts treated with 20 nM of smart pool siRNA. siRNA treatment had no effect on SMC1A expression. (D) SMC1B silencing was also confirmed by RT-qPCR following 24 hr siRNA treatment. *p < 0.05.
