Figure 3
From: SMC1B is present in mammalian somatic cells and interacts with mitotic cohesin proteins
Effects of SMC1B depletion in human primary fibroblasts.
(A) siRNA treatment had no effect on either chromosome number or morphology. (B) Diploid control metaphase. (C) γ-H2AX analysis in untreated control, mock and siRNA-treated cells irradiated with 5 Gy. The mean number of foci/cells for different repair times is shown. Error bars represent the SE from the analysis of 300 cells from three independent experiments. (D) Relative γ-H2AX phosphorylation levels determined after irradiation with 5 Gy by flow cytometry in control, mock and siRNA-treated cells for different repair times. Values for the fluorescence signal intensity were normalized to the value of the corresponding 0.5 h sample arbitrarily set as 100%. Both the number of foci/cell and fluorescence intensity was found to be statistically significant different 24 h after irradiation. *p < 0.05.
