Figure 4
From: SMC1B is present in mammalian somatic cells and interacts with mitotic cohesin proteins
SMC1B depletion causes gene dysregulation.
(A) In human primary fibroblasts the inhibition of SMC1B led to gene expression dysregulation, 92% of genes were downregulated (black) and 8% were upregulated (grey). (B) Chromosome 1 showed the highest number of downregulated genes (103) while Y chromosome the lower (8). No upregulated gene was found on chromosomes 21 and Y. (C) Microarray data was validated for five genes, COL11A1, GLDN, HBB, PCDHA1 and PROM1, by RT-qPCR. (D) The dysregulation of HOXD3, HOXD12, HOXB2, HOXB7, PCDHB5, PCDHB6, PCDHB19 and PCDHB was validated by RT-qPCR. *p < 0.05.
