Figure 4
Effect of IMB-LA on the Vpu-induced ubiquitination of BST-2.
HEK293T cells were co-transfected with pcDNA3.1-BST-2-HA, pcDNA3.1-Vpu and pCW7 (1:2:4). 24 h post-transfection, cells were treated with DMSO, 5 μM IMB-LA and 50 nM ConA for 24 h. Lysates were incubated with rabbit anti-HA antibody and then immunoprecipitated with protein A-agrose. The pre-IP lysates represented 1% of the IP input. The immunoprecipitated samples were analyzed by Western blot. The amount of ubiquitinated BST-2 was quantified using Image LabTM 2.0 software and normalized with that of the group expressing BST-2 and Vpu in the presence of DMSO. Full-length blots/gels are presented in Supplementary data.
