Figure 5
Effect of IMB-LA on cellular distribution of BST-2 in HeLa-Vpu cells.
HeLa (A) and HeLa-Vpu cells (B) were treated with DMSO or 5 μM IMB-LA. 24 h later, cells were incubated with rabbit anti-BST-2 and mouse anti-CD63 antibodies. Cells were then stained by fluor-conjugated secondary antibodies. Images were recorded using a spinning disc confocal microscope. Signals of BST-2 are pseudocolored as red, CD63 as green. (C) HeLa-Vpu cells were incubated with rabbit anti-BST-2 and mouse anti-LAMP1 antibodies. BST-2 signals are shown in red, LAMP1 in green. The left panels in (A,B) and (C) graphically represent the results of the Peason’s Correlation coefficients of the two channels in each set (Fig. 5, left panel).
