Figure 6
Heat shock experiment (measure intracellular amounts of Nα-acetyl-α-lysine at different times after subculturing mid-exponential phase cells to 42 °C) and sample point for RNA sequencing.
The culture of S. halodurans H3B36, which cultured in GMH medium containing 6% (w/v) NaCl at 30 °C, was split and shaken at 30 °C and 42 °C separately after reached to mid-exponential phase (an OD600 range of 0.5 to 0.6). The intracellular Nα-acetyl-α-lysine concentrations of cultures were begun to measure at different time (2, 4, 6, 8, or 20 h) after heat shock. Intracellular amounts of Nα-acetyl-α-lysine were calculated based on OD600 (i.e., μg per 1 mL per 1 OD600); (30 °C−30 °C)-NaAL represents subculturing to 30 °C (the control) and (30 °C−42 °C)-NaAL represents subculturing to 42 °C. The sample point marked in the figure represents the collection time for RNA sequencing.
