Figure 3
(A) Histogram of APD80 from all recording sites from 32 clusters. Solid lines show fits of the data by two Gaussian curves and dashed line indicates threshold used to divide groups of APs (shown in brown and blue). (B) Histogram of the principal component (PC), which is a linear combination of APD80 and APD30. Solid lines show fits of the PC data by two Gaussian distributions and dashed line indicates threshold used to divide groups of APs (shown in brown and blue). (C) Histogram of APD80 with waveform-based grouping, with colors showing different groups. Stacking of different colors indicate overlap in APD80 parameters between groups. (D–F) Average AP traces obtained by grouping APs based on APD80 threshold, PC of APD30 and APD80 and waveform analysis, respectively. (G) Scatter plot of APD80 and APD30 for all recorded APs. Dashed lines represent linear classification boundaries according to (1) threshold using APD80, (2) threshold using the principal component, which is a linear combination of APD30 and APD80 and (3) waveform analysis. (H) Comparison of APD80 between groups for the three different grouping methods. Colored bars correspond to groups of APs shown in (A–C). Waveform analysis resulted in groups with the most distinct AP differences, as indicated by the lowest DBI value.
