Figure 8
Effects of mitochondria and glycolysis on migration of other type of cells and intracellular mitochondrial localization in podocytes of adult mice.
In contrast to differentiated podocytes, 10 μM antimycin significantly reduced the migration of rat mesangial cells. In contrast, blocking glycolysis impaired cell migration in all cell types (A). Intracellular ATP level are likely reduced in the whole area of mesangial cell and its distribution are not altered by either antimycin or 10 mM 2DG/pyruvate (B). Bar, 50 μm. Electron micrograph showing that mitochondria (arrow) are predominantly located in the cell body and major processes, but not in foot processes, of adult mouse podocytes (C). Bar, 500 nm. Mitochondrial diameter (MD) is significantly greater than the length of foot processes (FPL) (D). Immunofluorescence shows that PFK is overlapped with nephrin (indicated by white arrow) in the mouse glomerulus (E). Schematic cartoons showing ATP distribution from mitochondria and glycolysis in cultured podocytes and the location of mitochondria in podocytes of mouse kidney are shown (F).
