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Figure 1

From: Competitive allele-specific TaqMan PCR (Cast-PCR) is a sensitive, specific and fast method for BRAF V600 mutation detection in Melanoma patients

Figure 1

Evaluation of Cast-PCR and Sanger analysis Limit of Detection (LOD).

For each of the BRAF mutations analyzed we determined the limit of detection by analyzing dilutions at different ratio of mutated (case 2 and case 23) and not mutated (case 40) clinical samples. (a) Cast-PCR plots for BRAFV600E mutation. (b) Cast-PCR plots for BRAFV600K mutation. (c) Sanger analysis of samples of different BRAFV600E:Not mutated ratio; (d) Sanger analysis of different BRAFV600K : Not mutated ratios.

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