Figure 2 | Scientific Reports

Figure 2

From: Competitive allele-specific TaqMan PCR (Cast-PCR) is a sensitive, specific and fast method for BRAF V600 mutation detection in Melanoma patients

Figure 2

The effect of melanin content on BRAF mutation detection.

(a) Case 6 did not show any mutation in the not purified sample by Sanger analysis as compared with the GC-purified correspondent sample. Cast-PCR plots show an amplification curve in both samples although Ct and ∆Ct values are higher in the not purified sample as compared with the GC purified. Cast-PCR results in the GC purified sample, were confirmed by molecular cloning. (b) Case 7 did not show any mutation even in the GC purified sample by Sanger analysis; on the other hand, Cast-PCR amplification was obtained exclusively upon GC-purification and this result was further confirmed by molecular cloning.

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