Figure 3
Intratumour and intertumor heterogeneity.
(a) For case 3 two different regions from the same section were analysed. Both regions showed a BRAFV600E mutation by Sanger sequencing as well as by Cast-PCR. However peak height in Sanger analysis was lower in sample A as compared to sample B, whereas Ct and ΔCt values were similar in both samples; (b) For case 24 the primary tumour and a synchronous metastatic lymphnode were analyzed. A BRAFV600K mutation was detected in the primary tumour but not in the metastatic lymphnode by both Sanger analysis and Cast-PCR, (c) For case 9 four different regions from the same section were analysed. Sanger sequencing detected the BRAFV600E mutation only in samples A and B, whereas Cast-PCR was able to detect the mutation in all four samples.
