Figure 4
From: MicroRNA-induced negative regulation of TLR-5 in grass carp, Ctenopharyngodon idella
TLR5 is a potential target of miRNA regulation.
(A) The alignment between miR142a-3p, miR-21, miR-223, cid-miRn-115, cid-miRn-131 and the 3′-UTR segment of tlr5. (B) CIK was exposed to FLG22 for 0 h, 24 h, 36 h and 48 h. The expression of Citlr5 in CIK was detected using real-time PCR. 18S rRNA expression was detected as internal control for mRNA. MiR-192 expression was detected as the internal control for miRNA. (C) MiRNA samples were extracted from different tissues, including kidney, spleen, intestine, liver, gill and blood. MiRNA expression was detected by qRT-PCR. MiR-192 was used as loading control. The data were obtained from three independent experiments (mean ± SD). *P < 0.05.
