Figure 5
From: MicroRNA-induced negative regulation of TLR-5 in grass carp, Ctenopharyngodon idella
cid-miRn-115 and miR-142a-3p overexpression changes the expression of Citlr5 and its downstream genes.
CIK received cid-miRn-115 and miR-142a-3p agomir at a dose of 50 nM for the indicated times. The relative mRNA expression of Citlr5 (A) and of TLR5 downstream genes, including Ciil-1β, Ciil-8 and Citnf-α (B and C) was detected using real-time PCR. 18S rRNA expression was used as internal control for mRNA. MiR-192 was detected as the loading control. The data were obtained from three independent experiments (mean ± SD). *P < 0.05.
