Table 1 PCR primer sequences used in this study.
From: MicroRNA-induced negative regulation of TLR-5 in grass carp, Ctenopharyngodon idella
Primer | Sequence (5′–3′) | Application |
|---|---|---|
Let-7a | tgaggtagtaggttgtatagtt | qRT-PCR |
Let-7e | tgaggtagtagattgaatagtt | qRT-PCR |
Let-7i | tgaggtagtagtttgtgctgtt | qRT-PCR |
miR-142a-3p | tgtagtgtttcctactttatgga | qRT-PCR |
miR-148 | tcagtgcattacagaactttgt | qRT-PCR |
miR-21 | tagcttatcagactggtgttggc | qRT-PCR |
miR-217 | tactgcatcaggaactgattgg | qRT-PCR |
miR-223 | tgtcagtttgtcaaatacccc | qRT-PCR |
miR-192 | atgacctatgaattgacagcc | qRT-PCR |
cid-miRn-115 | tgaaggccgaagtggaga | qRT-PCR |
cid-miRn-131 | tgcccgcattctccacca | qRT-PCR |
pEGPF-CiTLR5 | F: CCGGAATTCtgATGGGATTTACATTTATTCTGATCC | Overexpression |
R: CGCGGATCCgcTACTGATGTGTTTGCATGGACA | vector construction | |
Citlr5 | F: GAAGATAATCTACTTGGGTGAG | qRT-PCR |
R: GTCCGAGATGAAGAAGTTGTAG | ||
Ciil-1β | F: GCCAAGTAGCCGAATCACAGA | qRT-PCR |
R: AGAAGCCCAAGATATGCAGGA | ||
Ciil-8 | F: GACGCATTGGTAAACACA | qRT-PCR |
R: TAACCCAGGGAGCAGTAG | ||
Citnf-α | TCACGCTCAACAAGTCTCAG | qRT-PCR |
GAAGTAAATGCCGTCATCAG | ||
Ci18s rRNA | F: GGACACGGAAAGGATTGACAG | qRT-PCR |
R: CGGAGTCTCGTTCGTTATCGG |
