Figure 1 | Scientific Reports

Figure 1

From: The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models

Figure 1

Inactivation of the zebrafish prl gene.

(A) Schematic representation of wild-type (prl+/+, top) and two lines of TALEN targeted prl alleles (center and bottom). Exons are represented by red boxes with the targeted region in exon 1 shown as a blue box. TALEN arms are marked by an underline in the targeted region of the wild-type locus. (B) Schematic representation of the putative peptide of wild-type PRL (top) and the two mutated PRL peptides from the targeted alleles (center and bottom). (C) Genomic DNA PCR assay of 5 dpf larvae resulting from a cross between prl heterozygotes. (D) Western blot of the pituitary samples from wild-type (left) and prl-deficient (right) adults at 100 dpf. WT, wild-type; TALEN, transcription activator-like effector nuclease; PCR, polymerase chain reaction; Dpf, days post-fertilization.

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