Figure 5
After MNU administration, both the immunostaining of the r-transducin (A,C) and the PNA (B,D) showed progressive disappearance respectively. (E) The most resistant cone staining in the MNU administrated retinas was distributed in the ST quadrant. The cone staining density of the ST quadrant was greater than the other three quadrants at P5 and P7 and it eventually disappeared at P9 while these of other three quadrants disappeared by P7. Similaly, the rod staining density of ST quadrant was also the greatest one, while that of the IN quadrantwas was consistently the smallest one between P1 and P5. Quantification of immunostaining showed that the values of the four quadrants conformed to the following rule: ST > SN > IT > IN. (F) The rod immunostaining degenerated disproportionately to the cone immunostaining during the whole degenerated process (G). The rod immune staining density decreased by ~30% at P1 compared with the controls, while the cone staining degeneration was less terrible. Later at P3, both the rod and cone staining density reduced significantly: rod by ~57% and cone by ~37%. This inclination progressed till P5 and both staining density underwent substantial reduction. At P7, the rod staining almost vanished, while the cone staining was relatively retained (ANOVA analysis followed by post-hoc test, n = 10; Fig. (C–F) *P < 0.05, **P < 0.01 for differences between groups; Fig. (G) **P < 0.01 for differences between the rod and cone; ##P < 0.01 for differences compared with previous time point group).
