Figure 7
(A) The neuroretinal whole mounts were divided into quadrant patches by four incisions at 3, 6, 9 and 12 O’clock. (B) The Bax, Calpain-2, Caspase-3 expression were significantly up-regulated at P1 and peaked at P3. At P5, the expression levels of the three markers were down regulated, however, they were significantly higher than the normal control. At P7, the expression levels of the three markers recovered to normal. (C) The positional expression levels of these markers were assessed by qRT-PCR. The Bax expression in different retinal quadrants did not changed uniformly since P1. This disequilibrium among retinal quadrants became more obvious at P3 and P5. Similar positional disequilibrium was also found in the whole process of Calpain-2 up-regulation: initially at P1, the expression level in the IN quadrant was higher than the other three quadrants. This positional disequilibrium become more evidently at P3 and persisted throughout the up-regulation till P5. Intriguingly, no positional difference was found between any of the retinal quadrants throughout the whole process of Casepase-3 up-regulation (ANOVA analysis followed by post-hoc test, n = 8; Fig. (B) *P < 0.05, **P < 0.01 for differences compared with controls; #P < 0.05, ##P < 0.01 for differences compared with previous time point group; Fig. (C) *P < 0.05, #P < 0.01 for differences between groups).
