Figure 4

RPS23RG1overexpression mitigates oAβ-induced synaptic loss.

Cultured neurons were transfected with plasmids encoding human RPS23RG1 (hRps) and EGFP or EGFP alone and then exposed to oAβs or control Aβ_42-1. In some experiments, neurons were treated with additional DMSO or the PKA inhibitor H89. (A) Immunofluorescence images reveal localization of PSD-95 (red) and synapsin I (Syn; blue) clusters in neuronal dendrites. (B,C) Quantification of PSD-95 cluster (B) or PSD-95/Syn co-cluster (C) densities in neuronal dendrites (n_EGFP/Ctrl = 12, n_EGFP/Aβ = 10, n_hRps/Ctrl = 11, n_hRps/Aβ = 13). (D) Dendritic spines were visualized by transfected EGFP. (E) Quantification of spine density (n_{EGFP/Ctrl/DMSO} = 11, n_{EGFP/Aβ/DMSO} = 10, n_{hRps/Ctrl/DMSO} = 11, n_{hRps/Aβ/DMSO} = 13, n_{hRps/Ctrl/h89} = 10, n_hRps/Aβ/H89 = 10). Scale bar, 5 μm. Values were mean ± SEM (n.s., not significant. *^,#p < 0.05, **p < 0.01 one-way ANOVA with Dunnett’s multiple comparisons).