Figure 5
From: RPS23RG1 reduces Aβ oligomer-induced synaptic and cognitive deficits
Rps23rg1knockdown aggravates oAβ-induced synaptic loss.
(A) Representative images showing expression of PSD-95 (green) in cultured neurons transfected with Rps23rg1 or control siRNA and exposed to oAβs or control Aβ42-1. Only neuronal dendrites transfected with Cy3-tagged siRNAs (not shown) were selected for analysis. (B) Quantification of PSD-95 cluster densities showed that Rps23rg1 knockdown aggravated oAβ-induced synaptic loss. (C) Dendritic spines were visualized by transfected EGFP. (D) Graph summary showing that oAβ-induced spine loss was further enhanced by Rps23rg1 siRNA. Scale bar, 5 μm. Values were mean ± SEM (n = 6 cells per group from 3 cultures). #p < 0.05, **,##p < 0.01 one-way ANOVA with Dunnett’s multiple comparisons.
