Figure 5

VEGF and GCSF duplex assay.
Anti-VEGF and anti-GCSF antibodies were immobilized on different sensors with quadruplicates. The sample containing VEGF at 500 pg/ml and GCSF at 500 pg/ml was measured. The nanoparticles were added to the chip at 2 min. The extra distance between the nanoparticles and the surface still produced the same positive signals with respect to the baseline signal (before adding the nanoparticles). BSA signals were the same as the baseline after adding the nanoparticles. The error bars are the standard deviations of 4 identical sensor signals.