Figure 4 | Scientific Reports

Figure 4

From: Visualization of Assembly Intermediates and Budding Vacuoles of Singapore Grouper Iridovirus in Grouper Embryonic Cells

Figure 4

Knockdown of major capsid protein (MCP, ORF072).

(A) The sequence of the three peptides of MCP detected by mass spectrometry. (B) Morpholino-mediated knockdown of MCP resulted in reduced abundance for all three peptides. The comparison is based on the peak areas of identified peptides, calculated using Data Explored TM software (Applied Biosystems). The relative peak areas were converted to percentages, in which the peak area of corresponding peptides in the control sample was considered as 100%. (C) Knockdown of MCP reduced the yield of infectious particles measured by TCID50; (D-E) Following knockdown, the VAS contained deformed intermediates with few viral particles (D) or without any viral particles (E,F) Treatment with the control morpholino shows no negative effect on viral replication; scale bar = 1 μm.

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