Figure 1 | Scientific Reports

Figure 1

From: Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1

Figure 1

Transient expression of dual R proteins.

(a) Epitope-tagged constructs used in the transient expression. (b) Immunodetection of microsomal extracts from Nicotiana benthamiana leaves transiently expressed FLAG-RPS4 and/or Myc-RRS1 44 h after Agrobacterium infiltration. (c) Subcellular localisation of FLAG-RPS4, Myc-RRS1 and Myc-RRS1Δlz with immunoblotting. Transiently co-expressed FLAG-RPS4 and Myc-RRS1 or Myc-RRS1Δlz mainly localised in microsomal fractions 44 h after Agrobacterium infiltration. Microsomal (Micro) and nuclear (Nucl) fractions are 10- and 60-fold more concentrated, respectively, compared with the soluble (Sol) fraction. The degree of fraction enrichment was determined using antibodies against marker proteins (cytoplasmic soluble; anti-GAPDH, microsomes; anti BiP and nucleus; anti-Histone H3).

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