Figure 6 | Scientific Reports

Figure 6

From: Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1

Figure 6

EDS1 is required for dual R protein-induced hypersensitive (HR)-like cell death.

TRV:Vector or TRV:EDS1-silenced N. benthamiana leaves were infiltrated with Agrobacterium containing FLAG-gRPS4 and Myc-gRRS1 or Myc-gRRS1Δlz constructs. (a) HR-like cell death was observed at 3 dpi. The photograph was taken at 7 dpi. (b) Immunodetection of non-mutated RPS4, as well as RRS1 with a mutated LZ motif. Total proteins extracted from N. benthamiana leaves 44 h after Agrobacterium infiltration showed the transient co-expression of FLAG-RPS4 and Myc-RRS1 or Myc-RRS1Δlz. Immunodetection was performed with either anti-FLAG or anti-Myc antibodies.

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