Figure 2

Effect of cycloheximide on senescence features induced by several agents in human cells.

(a) HeLa cells were cultured in the presence of the agent indicated for 2 weeks, stained with the senescence-associated ß-galactosidase and subjected to photography. None, no addition; CHX, 0.15 μM cycloheximide; EdT, 2 mM thymidine; CPT, 7.5 nM camptothecin; AC, 0.3 μM aphidicolin. (b) TIG-7 cells were cultured in the presence of the agent indicated for 3 weeks and processed as in (a). None, no addition; CHX, 0.15 μM cycloheximide; EdT, excess 1 mM thymidine; CPT, 2 nM camptothecin; AC, 0.2 μM aphidicolin. (c) Numbers of HeLa cells stained with the senescence-associated ß-galactosidase were calculated (n = 3). (d) Numbers of TIG-7 cells stained with the senescence-associated ß-galactosidase were calculated (n = 3) as in (c). (e) Colonies of HeLa cells were stained with Coomassie Brilliant Blue. (f) Colonies of TIG-7 cells were stained with Coomassie Brilliant Blue. Bars denote means with a standard error of the mean (SEM). **P < 0.01 and ***P < 0.001, respectively, in comparison between -CHX and + CHX. Scale bars, 100 μm.