Figure 4
From: Restriction of protein synthesis abolishes senescence features at cellular and organismal levels
Effect of cycloheximide on protein content and cell volume in human cells.
(a) HeLa cells were cultured for 7 days as in Fig. 2 and total protein content per cell (n = 3-7) were determined. (b) TIG-7 cells were cultured for 7 days as in Fig. 2 and processed as in (a) (n = 3). (c) Mean volume of HeLa cells (n = 50) were determined. (d) Mean volume of TIG-7 cells (n = 50) were determined and processed as in (c). Bars denote means with a standard error of the mean (SEM). *P < 0.05, **P < 0.01 and ***P < 0.001 in comparison between -CHX and + CHX.
