Figure 5
From: Restriction of protein synthesis abolishes senescence features at cellular and organismal levels
Western blot analysis of ERK1/2 in HeLa cells.
(a) Cells were cultured in the presence of the agents indicated for 1 day and cell extracts were subjected to western blot analysis using specific antibodies to total and phosphorylated ERK1/2 as described previously44. None, no addition; CHX, 0.15 μM cycloheximide; EdT, 2 mM excess thymidine; CPT, 7.5 nM camptothecin; AC, 0.3 μM aphidicolin. (b) Cells were cultured for 5 days and processed as in (a). The full-length images are shown in Supplementary Fig. 7.
