Figure 1
From: Altered brain energetics induces mitochondrial fission arrest in Alzheimer’s Disease
Mitochondrial morphology in CA1 hippocampi of NTG and FAD mice visualized using standard TEM and super-resolution immunofluorescence.
(a) Mitochondrion in a neuropil in brain tissue of a NTG mouse. (b–f) Micrographs of mitochondrial profiles in the brain tissue of APP (b), 3xTgAD (c,d) and APP/PS1 (e,f) mice. (d) Consecutive serial sections of hippocampi from 3xTgAD mouse showing mitochondrial fission. (g–j) Membrane connections that contain mitochondrial matrix (g,h) or are devoid of matrix components (i,j) observed in APP/PS1 mice. (k,l) Mitochondria in brain tissue of NTG (k) or APP/PS1 (l) mice observed using Tom20 antibody (green) and a super-resolution fluorescence microscopy. Low magnification images on the right are co-stained with Hoechst (blue) to define nuclei. Scale bar, 2 μm. Three to five mice per each genotype were examined. All mice were females 40 weeks of age. Ten random sections with ~100 mitochondria were examined by blinded investigator for each brain tissue.
