Figure 5
Aim23 specifically associates with the small subunit of mitochondrial ribosome.
Mitochondria were lysed with 1% n-dodecyl β-D-maltoside in the presence of 100 mM KOAc leading to the dissociation of mitochondrial ribosomes into subunits and the lysate was separated by centrifugation on a linear sucrose gradient as per Kehrein and colleagues57. Aconitase (Aco1) was used as soluble protein control and it stays in the top of the gradient. Separated ribosomal subunits migrate into the gradient and are detected using antibodies against small subunit protein Mrps5 and large subunit protein Mrpl36. T is a loading control corresponding to 10% of the starting material applied on the gradient, Mw is molecular weight in kDa.
