Figure 2

Replication origins are separated within the S. pombe nucleus.

Contour maps of the locations of early and late firing replication origins³ in ensembles of contact-restrained genome structures that were generated as described in Fig. 1. (A) Early firing origins were central and late firing replication origins were located towards the periphery. Late firing origins were more centrally located in models derived from cells grown on undefined media¹⁰. (B) High efficiency origins fire in most cell cycles³ and are preferentially located toward the nuclear center and about the SPB. By contrast, low and medium efficiency origins³ are localized towards the nuclear periphery and nucleolar boundary, respectively. Low efficiency origins are more centrally located in cells grown in undefined media.