Figure 3
Prussian Blue (PB)-stained sections in the abdomens of the 1st–5th instar nymphs, macropterous and brachypterous female and male adult BPHs, Nilaparvata lugens.
PB-reactive products of high concentrations of iron appear as small pale-blue spherules (arrows) or dark-blue aggregates (arrows). Bar = 10 μm. More than 130 sections were used for counting for each insect stage, sex and wing form. The sections were evaluated under an Olympus microscope BX51 (Olympus, Japan) with a DP70 digital camera.
