Figure 8
Relative amounts of magnetic particles in the abdomens of BPH, Nilaparvata lugens.
Prussian Blue staining was used to identify the magnetic particles in the section of abdomens. More than 130 sections were used for counting for each insect stage, sex and wing form. The sections were evaluated under an Olympus microscope BX51 (Olympus, Japan) with a DP70 digital camera. The dark blue stained dots as well as or zones in the images photographed by the digital camera were scored for calculating the relative amount of magnetite using ImageJ software (version 1.47). The mean grayscale values calculated from the dark blue zones of the sections of 3rd instar female insects that contained the lowest amount of magnetic particles were used as a standard (100 percentage ratio) to compare with other values to obtain a percentage ratio to quantify the relative amount of magnetic particles in the abdomens of other instar or adult insects. Different lowercase letters (a–d) indicate significant differences among the tested BPH by LSD test at P < 0.05; nd - not detected.
